Evaluation of in vitro anti-acne activity of methanolic extracts of Cyperus rotundus and Nymphaea nouchali

Background: The objective of this study was to evaluate in vitro anti-acne activity against Staphylococcus aureus (S. aureus), epidermidis epidermidis), and Escherichia coli (E. coli) using the agar well diffusion method. Methods: Air-dried ground, matured rhizomes Cyperus rotundus (CR), petals pollen Nymphaea nouchali (NN) were macerated methanol. resultant extracts concentrated a rotary evaporator. anti-bacterial performed (ATCC 25923), (ATCC12228), 25922) method, gentamicin used as positive control. whole experiment done triplicates. Results: data obtained from dose-response curves, methanolic staphylococcus (IC50=819.2 µg/ml), pollens (IC50=787.7 µg/ml) combination (IC50=813.6 exhibited highest potency. A strong statistically significant correlation between zone inhibition concentrations all plant extracts. p value less than 0.05 (p<0.05) R2 values around 1. Conclusions: maximum potency equivalent observed S. aureus, epidermidis, E. at concentration 15625 µg/ml combination. present might bring progress treatment acne herbs developing herbal formulations for safe effective management disease.